Evaluation of the semen swim-up method for bovine sperm RNA extraction
نویسندگان
چکیده
منابع مشابه
Evaluation of the semen swim-up method for bovine sperm RNA extraction.
Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were purified by the swim-up method. RNA extraction was carried out using the ...
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OBJECTIVE To study the impact of sperm swim up technique of seminal processing for the purpose of intrauterine insemination (IUI) in couples presenting with subfertility. METHODS Hundred and twenty-one couples presenting with subfertility underwent 281 cycles of IUI in Combined Military Hospital Kharian, Lahore and PNS SHIFA Karachi from June 2002 to March 2005. Men had prior semen analysis t...
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Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach is more effective. The aim of the present study was to compare the effects of these two methods on sperm telomere length (STL). A total of 150 normozoospermic subject...
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OBJECTIVE Several methods for evaluating semen quality have been developed in addition to traditional semen analysis. Most of these methods are very complex and require expensive technical equipment as well as special knowledge to be performed. The aim of this study was to establish an easy and cost-effective sperm penetration test for bovine semen using artificial media. Additionally a standar...
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Objective: To compare the effectiveness of carbon dioxide and carbon dioxide-free incubation system in extracting motile, morphologically normal and chromatin intact sperm populations from the individual semen samples. Materials and Methods: Semen samples were obtained from normozoospermic men presenting for infertility evaluation. Individual samples were divided into two equal aliquots for spe...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2016
ISSN: 1676-5680
DOI: 10.4238/gmr.15027713